全文获取类型
收费全文 | 6552篇 |
免费 | 352篇 |
国内免费 | 10篇 |
专业分类
林业 | 325篇 |
农学 | 346篇 |
基础科学 | 67篇 |
997篇 | |
综合类 | 443篇 |
农作物 | 289篇 |
水产渔业 | 256篇 |
畜牧兽医 | 3341篇 |
园艺 | 144篇 |
植物保护 | 706篇 |
出版年
2021年 | 47篇 |
2020年 | 105篇 |
2019年 | 88篇 |
2018年 | 118篇 |
2017年 | 141篇 |
2016年 | 123篇 |
2015年 | 95篇 |
2014年 | 126篇 |
2013年 | 344篇 |
2012年 | 262篇 |
2011年 | 329篇 |
2010年 | 253篇 |
2009年 | 217篇 |
2008年 | 306篇 |
2007年 | 270篇 |
2006年 | 252篇 |
2005年 | 250篇 |
2004年 | 228篇 |
2003年 | 205篇 |
2002年 | 232篇 |
2001年 | 198篇 |
2000年 | 187篇 |
1999年 | 174篇 |
1998年 | 79篇 |
1997年 | 62篇 |
1996年 | 69篇 |
1995年 | 52篇 |
1994年 | 62篇 |
1993年 | 76篇 |
1992年 | 109篇 |
1991年 | 116篇 |
1990年 | 118篇 |
1989年 | 109篇 |
1988年 | 83篇 |
1987年 | 75篇 |
1986年 | 67篇 |
1985年 | 74篇 |
1984年 | 81篇 |
1983年 | 62篇 |
1982年 | 63篇 |
1981年 | 45篇 |
1980年 | 50篇 |
1979年 | 51篇 |
1978年 | 54篇 |
1977年 | 49篇 |
1976年 | 53篇 |
1975年 | 43篇 |
1974年 | 52篇 |
1971年 | 49篇 |
1969年 | 53篇 |
排序方式: 共有6914条查询结果,搜索用时 15 毫秒
21.
22.
Increasing interest in a longer preservation of diluted boar sperm raises questions in the field concerning the choice of the extender. The aim of this study was to evaluate the longevity of boar sperm extended in currently used commercial semen extenders. Three long-term extenders and two short-term extenders were compared for different semen quality parameters that can be assessed under routine laboratory conditions. Sperm morphology, motility, pH and bacteriological contamination were investigated during a 7-day period. The number of dead spermatozoa did not differ significantly among the extenders (p > 0.05). Sperm motility was not only related with storage period but most of all with pH, especially in long-term extenders. Differences between the different extenders were prominent (p < 0.05); the sperm preserved in only one long-term extender showed good motility during the whole test period. In all cases, the pH of the extended semen increased by 0.3-0.5 in the first days of storage and was significantly correlated with a decrease in motility. Bacteriological quality had no significant influence on motility or pH of the semen. In conclusion, we can state that in both short-term extenders and in only one long-term extender, sperm longevity, as evaluated by the parameters used in this study, was sufficient during the preservation period. To preserve the quality of diluted boar semen during long-term storage, the choice of the long-term extender is important. In addition, the monitoring of the pH of extended boar semen in our study emphasizes the importance of the buffering capacity of semen extenders. 相似文献
23.
Hieu Van DONG Maya SUZUKI Hitoshi TAKEMAE Dulamjav JAMSRANSUREN Sachiko MATSUDA Hiep Dinh NGUYEN Tetsuya MIZUTANI Yohei TAKEDA Haruko OGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1543
In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV) genome-positive cattle sera and bovine coronavirus/infectious bovine rhinotracheitis virus-spiked cattle nasal swabs. In addition, the subgenotyping of BVDV strains detected in Tokachi Province in Japan during 2016–2017 was performed. Results showed that most of these BVDV strains belonged to subgenotype 1b, while few strains belonged to subgenotypes 1a and 2a. This study showed the high applicability of automatic nucleic acid extractors in extracting multiple viral genomes and the dominant subgenotype of BVDV in Tokachi. 相似文献
24.
25.
Eckhard Koch Annegret Schmitt Dietrich Stephan Carola Kromphardt Marga Jahn Hermann-Josef Krauthausen Gustaf Forsberg Sigrid Werner Tahsein Amein Sandra A. I. Wright Federico Tinivella Maria L. Gullino Steven J. Roberts Jan van der Wolf Steven P. C. Groot 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(1):99-112
The current study was initiated to evaluate the efficacy of physical methods (hot water, aerated steam, electron treatment) and agents of natural origin (resistance inducers, plant derived products, micro-organisms) as seed treatments of carrots for control of Alternaria dauci and A. radicina. Control of both Alternaria species by seed treatment with the resistance inducers was generally poor. Results were also not satisfactory with most of the formulated commercial micro-organism preparations. Based on the average of five field trials, one of these, BA 2552 (Pseudomonas chlororaphis), provided a low but significant increase in plant stand. Among the experimental micro-organisms, the best results were obtained with Pseudomonas sp. strain MF 416 and Clonostachys rosea strain IK726. A similar level of efficacy was provided by seed treatment with an emulsion (1%) of thyme oil in water. Good and consistent control was generally achieved with the physical methods aerated steam, hot water and electron treatment. Aerated steam treatment was, apart from the thiram-containing chemical standard, the best single treatment, and its performance may at least partially be due to extensive pre-testing, resulting in dosages optimally adapted to the respective seed lot. In some of the experiments the effect of the hot water treatment, which was tested at a fixed, not specifically adapted dosage, was significantly improved when combined with a Pseudomonas sp. MF 416 or C. rosea IK726 treatment. The results are discussed in relation to the outcome of experiments in which the same seed treatment methods and agents were tested in other seed-borne vegetable pathosystems. 相似文献
26.
27.
Nadezhda E. Ustyuzhanina Maria I. Bilan Andrey S. Dmitrenok Eugenia A. Tsvetkova Sofya P. Nikogosova Cao Thi Thuy Hang Pham Duc Thinh Dinh Thanh Trung Tran Thi Thanh Van Alexander S. Shashkov Anatolii I. Usov Nikolay E. Nifantiev 《Marine drugs》2022,20(6)
Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods. Both FCSs were shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n bearing sulfated fucosyl branches at O-3 of every GlcA residue in the chain. These fucosyl residues were different in pattern of sulfation: FCS-BA contained Fuc2S4S, Fuc3S4S and Fuc4S at a ratio of 1:8:2, while FCS-HS contained these residues at a ratio of 2:2:1. Polysaccharides differed also in content of GalNAc4S6S and GalNAc4S units, the ratios being 14:1 for FCS-BA and 4:1 for FCS-HS. Both FCSs demonstrated significant anticoagulant activity in clotting time assay and potentiated inhibition of thrombin, but not of factor Xa. FS-BA-AT was shown to be a regular linear polymer of 4-linked α-L-fucopyranose 3-sulfate, the structure being confirmed by NMR spectra of desulfated polysaccharide. In spite of considerable sulfate content, FS-BA-AT was practically devoid of anticoagulant activity. FS-HS-AT cannot be purified completely from contamination of some FCS. Its structure was tentatively represented as a mixture of chains identical with FS-BA-AT and other chains built up of randomly sulfated alternating 4- and 3-linked α-L-fucopyranose residues. 相似文献
28.
禁止在家禽饲料中添加抗生素对于减少对人类健康有潜在威胁的耐药性细菌几乎没有作用。来自美国的一个科研小组通过研究得出了以上惊人的结论。 相似文献
29.
饲料加工对家禽生产性能的影响 总被引:1,自引:0,他引:1
菊粉(inulin)被认为是饲用抗生素潜在的替代物.在实际应用中不存在任何技术性问题.精细粉碎的全脂大豆和菊粉混合物被证明是幼龄肉火鸡开食阶段一种非常有前途的"提高质量"工具. 相似文献
30.
由鞭毛组织滴虫(histomonas meleagridis)引起的组织滴虫病(或黑头病)是一种老病新发的禽病,此病除侵害盲肠和肝脏之外,还会侵害其他组织.此病的有效防治药物被禁用促使人们对此进行新的研究,并且已找到了可防止家禽爆发此病的新方法. 相似文献